Composite

Part:BBa_K4644014:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-07)


ygay-50trc-gdhA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 36
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 36
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 36
    Illegal BamHI site found at 1407
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 36
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 36
    Illegal AgeI site found at 645
    Illegal AgeI site found at 1001
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we used CRISPR-Cas9 technology to target the ygaY locus in the genome for integration.


Source

E.coli

References

Lightfoot D A, Mungur R, Ameziane R, et al. Improved drought tolerance of transgenic Zea mays plants that express the glutamate dehydrogenase gene (gdhA) of E. coli[J]. Euphytica, 2007, 156: 103-116.

Pagliarulo C, Salvatore P, De Vitis L R, et al. Regulation and differential expression of gdhA encoding NADP‐specific glutamate dehydrogenase in Neisseria meningitidis clinical isolates[J]. Molecular microbiology, 2004, 51(6): 1757-1772.